DDPH对多种介质所致豚鼠离体肠道平滑肌收缩的影响

Inhibitory effects of DDPH on contraction ofisolated ileum of guinea pigs induced by some mediators

目的研究1_(2,6_二甲基苯氧基)_2_(3,4_二甲氧基苯乙氨基)丙烷盐酸盐(DDPH)对多种介质引起的豚鼠离体肠道平滑肌收缩的影响。方法肠道平滑肌实验法。结果不同剂量的DDPH可非竞争性抑制磷酸组胺、氯化乙酰胆碱及Ca2 + 的作用,使量效曲线非平行右移 ,最大反应压低 ,其 pD2’值分别为4 0、3 8、3.9。DDPH还可抑制K+ 所致肠道平滑肌收缩 ,但作用较维拉帕米弱 ,IC50 值分别为2 7μmol·L-1 和0 7μmol·L-1。结论DDPH通过非竞争性Ca2 + 拮抗作用使豚鼠回肠平滑肌松弛。

Aim To investigate the effects of 1-(2,6-dimethylphenoxy)-2-(3,4-dimethoxypheny- lethylamino) propane hydrochloride (DDPH) on contraction of isolated ileum of guinea pigs induced by some mediators. Methods The routine methods for experiment on contraction-effect of the isolated ileum were emplayed.Results Histamine, acetylcholine and CaCl2 separetely caused a significant cumulative concentration-related effect on ileum constraction with the pD2 values being 7.7?9.8 and 5.3 respectively. After being incubated with DDPH of different dosage for 15 min, the responses elicited by Hist. ACh and CaCl2 were depressed and the pD2' values were 4.0, 3.8 and 3.9 respectively.Cumulative concentration of DDPH and verapamil resulted in a concentration dependent relaxing effect on contraction of ileum induced by K+ 60 mmol·L-1 with the IC50 values being 2.7 μmol·L-1 and 0.7 μmol·L-1. Conclusion DDPH has significant relaxing effect and a calcium antagonistic effect on ileum.