三七总皂甙对大鼠局灶性脑缺血的保护作用

Protective effects of punax notojinseg on local cerebral ischemia in rats

目的 研究三七总皂甙(PNS)对大鼠局灶性脑缺血的保护作用.方法 阻断大鼠大脑中脉造成可逆性脑缺血模型,观察PNS对缺血脑组织的保护作用.结果PNS 20、40mg·kg^(-1)·d^(-1)×10d能显著改善大鼠MCAO后的神经功能障碍,缩小脑梗塞面积,减轻脑水肿,并能降低缺血脑组织的总钙含量,明显提高SOD活力,降低MDA含量.结论 PNS对局灶性缺血脑组织具有保护作用,其机理推测与降低脑组织钙含量及抗氧自由基损伤有关.

Aim To study the protective effects of punax notojinseg (PNS) on local cerebral is-chemia in rats. Methods Local cerebral ischemia in rats was induced by occlusion ofthe middle cerebral artery (MCAO) without craniectomy. Results PNS in the dosesof 200 and 400 mg· kg-1·d-1 ×10 d significantly decreased the infarct size and brainedema and ameliorated neurologic deficits in rats subjected to MCAO fpr 2 or 24hours. The same dosage of PNS could markedly increase the activity of superoxide dis-mutase (SOD) and decrease the concentration of malondialdehyde (MDA) and Ca2 + inthe brain.Conclusions Declining free radicals and decreasing Ca2+ content in brainmight be one of the important factors involved in the anti-cerebral ischemia effect ofPNS.