抗生素及抗凝药对小鼠腹膜影响实验研究

Experimental study of antibiotics and anticoagulants effection on mouse peritonaeum

目的 观察抗生素及抗凝药对腹膜的影响，探讨腹膜透析相关硬化性腹膜炎的因素。方法 用小鼠建立腹膜透析实验模型，模拟腹膜透析常用的药物注入小鼠腹腔内，通过大体解剖和光镜组织学观察各种药物在不同时间腹膜结构的变化。结果 丁胺卡那霉素、头孢拉啶、头孢呋新（西力欣）、环丙沙星、肝素及尿激酶在用药2-4wk均使腹膜间皮细胞丧失，炎症细胞浸润，纤维细胞增生；在用药6-8wk更为显著。停药后这些改变不能完全恢复。结论 腹膜透析常用的抗生素及抗凝药均有致腹膜硬化的作用。但程度不一，这对临床选药及腹膜功能的保护具有重要意义。

Aim The effects of antibiotics and anticoagulants on mouse peritonaeum were ob-served to explore the factor of the peritoneal dialysis related sclerosing peritoni-tis. Methods The experimental models of peritoneal dialysis were established in miceby infusing different kind of drugs to the peritoneal cavity and the changes of the peri-toneal membrane for each drug at different time were observed by the autopsy and lightmicroscope for several weeks. Results Amikacin, Cefradine, Zinacef, Ciprofloxacin,Heparin and Urokinase could induce sclerosing changes of peritoneal membrane such asloss of peritoneal mesothelum infiltration of inflammatory cells and of proliferation fibrecell.These changes were irreversible after the drugs were stoped.Conclusion Thedrugs commonly used in peritoneal dialysis may in different degree result in peritonealsclerosis.