碱性成纤维细胞生长因子在乳腺良恶性病变细胞中的分布特征及其微血管密度的关系

The located features of basic fibroblast growth factor in cells of the benign and malignant hyperplasia lesions in breast and related to microvessel density

目的 :研究碱性成纤维细胞生长因子 (bFGF)在乳腺癌细胞中的表达特征 ,寻找有助于诊断乳腺癌的生物学标记 .方法 :收集乳腺腺病、乳腺纤维腺瘤和乳腺癌等的手术切除标本各 2 2例 ,常规石蜡包埋切片 (4μm) ,分别做HE染色和用ABC法进行bFGF(1∶2 0 0 )、血管标记抗体CD34(1∶10 0 )免疫组织化学染色 ,参考Weidner和Maeda方法进行微血管密度 (MVD)测定 .结果 :在乳腺腺病和乳腺纤维腺瘤中的肌上皮细胞、导管上皮细胞、血管内皮细胞、血管壁平滑肌细胞和纤维母细胞等均表达bFGF ,其阳性产物主要位于胞浆 ,肌上皮细胞和导管上皮细胞的阳性产物部分见于胞核 ,但乳腺癌细胞的阳性产物主要位于胞膜 ,少数位于胞浆 ;胞核阴性 ,与良性增生性病变形成鲜明的对照 .2 2例乳腺癌bFGF膜阳性者 17例 (占 77 3% ) ,其MVD =90 5 6± 2 3 6 2 .肿瘤直径 4cm(10 /2 2例 )时 ,MVD增高达 93 19± 2 0 75 .乳腺腺病MVD =4 2 0 5± 12 2 6 ,乳腺纤维腺瘤MVD =4 3 14± 18 2 1,与乳腺癌相比有显著差异 (P <0 0 1) .结论 :1)bFGF免疫染色阳性产物在乳腺癌细胞中主要分布在胞膜 ,在良性增生性病变细胞中主要位于胞浆和胞核 .乳腺癌的MVD明显高于良性增生性病变 .2 )质量好的免疫染色、bFGF反应产物的分布特征 。

Aim:To study the expression features of basic fibroblast growth factor (bFGF) in benign and malignant breast hyperplasia lesion and looking for a biological mark for diagnosis of the breast cancer. Methods: Tissues of 66 cases including the respective 22 cases of breast carcinoma, fibroadenoma and adenosis were fixed and embedded by routine pathologic technique and serial sections (4 μm). Immunostains of bFGF (1∶200) and CD34 (1∶100) were conducted by ABC method. The microvessel density (MVD) was estimated with Weidner and Maeda Method. Results: bFGF positive products, in the fibroadenoma and adenosis were mainly in the cytoplasm and nuclei of the cells of myoepithelial, hyperplasia ductal epithelial and endoepithelial of vessel, but mostly (17/22, 77 3%) occurring on the membrane of the cells in the breast carcinoma, MVD of which was 90 56±23 62 and greater than that of both fibroadenoma (43 14±18 21) and adenosis (40 05±12 26) ( P <0 01). The MVD increased up to 93 19±20 75 when the diameter of carcinoma (10/22 cases) was over 4 cm in diameter. Conclusion:The positive products of bFGF immunostain in breast carcinoma was mainly on the carcinoma cell membrane and significantly different from that mostly in the cytoplasm and nuclear of cells of the breast benign hyperplasia lesions. The located features of bFGF expression combined with the MVD may be useful to predict the tendency of malignant transformation of benign hyperplasia lesions or to diagnosis of breast carcinoma.