摘要
UDS试验是检测DNA损伤的短期试验之一.用UDS试验检测三种不同来源的氰戊菊酯,氰戊菊酯№1和№2浓度在5×10^(-8)～10^(-5)mol/L,有或无代谢活化系统时,都无诱发UDS作用.氰戊莉酯№3在不和体外活化系统,浓度为5×10^(-5)mol/L时结果为阳性,并能获得剂量效应关系.加有大鼠肝微粒体制剂时,氰戊菊酯№3浓度在5×10^(-8)～10^(-5)mol/L,无诱发UDS作用.比较三种氰戊菊酯制品的纯度后认为,№3的DNA损伤作用可能是样本中之杂质所引起.
UDS assay is one of the short-term tests to detect DNA damage. Fenvalerate from three different sources has been tested by UDS assay to confirm that fenvalerates No.1 and No.2 have no UDS-inducing effect at the concentration ranges of 5 × 10^(-8) to 5 × 10^(-5) mol/L either with or without the metabolic activation system. Fenvalerate No.3 gives a positive result at the concentration of 5 × 10^(-5)mol/L in the absence of the metabolic activation system and a dose-response relationship has been discovered. Its UDS-inducing effect would disappear in the presence of rat-liver microsomal preparation. The comparison of the purity of the three fenvalerate samples suggests that the DNA damaging effect by fenvalerate No.3 might be caused by its impurity.
