诊断血行播散型肺结核的细菌学方法

Bacteriological method in diagnosing hematogenous pulmonary tuberculosis

目的 探讨一种诊断血行播散型肺结核(以下简称Ⅱ型结核)的细菌学方法。方法 抽取Ⅱ型结核病患者静脉血,采用试管漂浮法直接检测静脉血中的结核杆菌,同时采用PCR(多聚酶链反应)和PPD-T(含吐温标准制结核菌素)、PPD-B(标准纯蛋白衍生物-巴蒂杆菌)试验做对比试验,并设立正常对照组。结果 32例Ⅱ型结核患者中,除2例外均检测出结核菌,正常对照组均为阴性。结论 此法对Ⅱ型结核具有重要的鉴别诊断价值。

[Objective]To study a new bacteriological method of diagnosing hematogenous pulmonary tuberculosis (HPT) . [Methods] The mycobacterium tuberculosis (TB) in venous blood of HPT' s patients was detected with the tube floating method. PCR and PPD - T, and PPD - B were applied in the contrasting experiment. Meanwhile, normal control groups were built. [Results] TB was found in the venous blood of 30 patients out of 32. While that of the normal control group was negative. [ Conclusion] This method is of much value in differential diagnosing of HPT.