油菜与诸葛菜的几种分子标记比较研究

Comparison study on several molecular markers between rapeseed and Orychophragmus violaceus

利用分子标记 (随机引物、简单重复序列、微卫星DNA重复序列、rDNA -ITS)比较研究了白菜型油菜与诸葛菜的亲缘关系。RAPD分析表明 ,诸葛菜与白菜型油菜PCR产物相似性只有 10 %左右 ;简单重复序列 (SSRs)标记中引物B .n .12A的PCR产物表明诸葛菜与白菜型油菜有明显差异 ,诸葛菜没有扩增产物 ,白菜型油菜具有明显的扩增产物 ;引物ITS4-IT5的PCR产物也有明显的差异。这些结果说明白菜型油菜与诸葛菜的亲缘关系不是很近。结合其他学者的工作 ,讨论了甘蓝型油菜。

Relative relationships between rapeseed and O.violaceus were studied by using molecular markers (random primers, simple sequence repeat, microsatellites DNA sequence repeat, ribosome DNA-ITS region). Through RAPD analysis, about 10% of similarity for PCR products between B.campestris and O.violaceus were found. Primer pair of B.n.12A in simple sequence repeats produced some different outcome, O.violaceus without amplified products and B.campestris with one band. The difference from PCR products of primer pair ITS 4-IT 5 between B.campestris and O.violaceus were found, O.violaceus with 1 band and B.campestris with 4 bands. Those results showed that relative relationships between B.campestris and O.violaceus couldn't near. Combined with other scholar works, relative relationships among B.napus , B.juncea and O.violaceus were discussed.