供体PGCs在鸡-鸭嵌合体胚胎性腺外的发育

Extragonadal Development of Donor PGCs in Chicken-Duck Chimeric Embryos

通过微注射法将鸡原始生殖细胞 (PGCs)注入到鸭的胚盘下腔中 ,用鸡 W染色体 DNA探针通过原位杂交的方法对供体 PGCs在嵌合体胚胎性腺外的发育作了研究。结果表明 ,所取的 4 8枚胚胎中 ,有 18.8%的脑内和 2 3.

Chicken PGCs from a line of White Leghorns were microinjected into Maya duck blastoderms at stage Ⅹ.Extragonadal development of donor PGCs in chicken duck chimeric embryos were studied by in situ hybridization with W chromosome specific DNA probes.In approximately 18 8% and 23 9%,respectively,of species from 48 chimeric embrys the chicken PGCs migrated into the brains and the neural tubes.