大鼠视网膜组织中线粒体的体视学定量研究

Study on Stereologic Quantity of Mitochondrion in Rat's Retinal Tissues

目的 研究线粒体在正常大鼠视网膜组织的变化特点及规律。方法 采用SD大鼠视网膜组织 ,用电镜和图像分析仪的方法 ,对视网膜组织中线粒体的进行体视学定量分析。结果 视网膜内节、外网状层及内网状层线粒体空泡的体积密度、表面积密度及内节与内网状层线粒体空泡的表面积与体积比差异均有显著性 ,并且线粒体的形状因子差异性较为明显 ,并按内节→外网状层→内网状层的顺序递增 ,从长杆状、椭圆形向圆形靠近。结论 视网膜内节线粒体产生的能量大 ,感光细胞所需能量多 ,功能活跃。视网膜外网状层线粒体产生的能量较内节少 ,双节细胞所需能量较感光细胞少 ,功能活动较内节差。视网膜内网状层线粒体产生的能量较少 ,表明神经节细胞活动不太活跃。

Objective: Study the characteristics and laws of changing mitochondrion in rat's normal tissues. Method: Use SD rat retinal tissues to carry out a quantitative analysis of mitochondrial stereology in retinal tissues with electromicroscope and image pattern analysis. Result: There is an apparent difference between the bulk and surface area density of mitochondrioal vacuoles in retina's internal segment, both external and internal reticular layers and the surface area and bulk ratio of mitochondrioal vacuoles in internal segment and internal reticular layer. There is also the significance of difference in mitochondrion shape factor, increasing progressively from internal segment to external and internal reticular layer, nearing circle from rod and oblong. Conclusion: The greater the mitochondrial energy in retina's internal segment is, the more the photosensory cell needs. So is the active function. Nevertheless, the mitochondrial energy in retina's external reticular layer is less the that in internal segment, the energy needed by sectioned cell is less than that by photosensory cell and its function is poorer. The less energy produced by mitochondrion in retina's internal reticular layer shows the inactivity of the ganglion cell.