油松雌性不育系(28号无性系)的RAPD分析

RAPD analysis in female sterility (Clone 28 ) of Pinus tabulaeformis Carr.

辽宁兴城油松种子园中的 2 8号无性系是雌性不育变异体 .为了探索不育原因 ,揭示与育性相关的分子机制 ,该实验运用RAPD技术对 2 8号雌性不育系及部分可育系进行分析 ,目的在于寻找与 2 8号不育系遗传背景最相近的可育系 ,为进一步研究提供选材依据 .实验结果表明 ,5 9个 10碱基的随机引物共扩增出 2 36个条带 ,其中具有多态性的条带有 4 2个 ,占 17 8% ,证明供试油松无性系间遗传变异性较小 .用NTSYS2 0软件包进行结果分析 ,发现可育系中15号无性系与 2 8号不育系遗传距离较近 ,此组材料可用作研究油松雌性不育分子机制的对比材料 .用S37号引物从2 8号雌性不育系基因组扩增出一条分子量为 75 0bp的特异DNA片段 .

Clone 28 of Pinus tabulaeformis Carr. is a female sterility variation,which was found in the Xingcheng Seed Garden of Liaoning Province.To explore the molecular mechanism of the female sterility,find the female fertility clone that is similar to Clone 28 in the genetic background,Clone 28 and the female fertility clones of Pinus tabulaeformis Carr. were investigated by random amplified polymorphic DNA (RAPD) analysis.The results revealed that 59 random primers with 10 mers produced 236 distinctive bands in total, 42 of which were polymorphic bands,the percentage was 17 8%,which proved that the genetic variation level in Pinus tabulaeformis Carr. was relatively low. The genetic distance was calculated and the dendrogram were generated by using the NTSYSpc 2 0 software. The results revealed that Clone 15 of the female fertility was most similar to the female sterility (Clone 28).These two clones are to be used for the molecular mechanism study of the female sterility as a pair of comparison materials. The random primer S37 produced one 750bp band from the genome of Clone 28,which was characterized by the female sterility clone.