高效液相色谱法测定不同部位两面针原植物中L-芝麻脂素和L-细辛脂素的含量

Determination of L-sesamin and L-asarinin in Zanthoxylum(Roxb.) DC. by high performance liquid chromatography

目的 建立两面针原植物中L-芝麻脂素和L-细辛脂素的测定方法,并测定两面针不同部位两者的含量。方法 采用高效液相色谱法（HPLC）,色谱柱为HYPERSIL BDS C18,流动相为乙腈:水（50:50）,检测波长287nm。结果 L-芝麻脂素和L-细辛脂素的平均回收率和相对标准差分别为100.13％、1.61％和101.24％、1.46％。结论 此法可用于两面针的质量控制。两面针原植物根部的被测成分含量最高。

Objective To establish a method for determining the content of L-sesamin and L-asarinin indifferent parts of Zanthoxylum (Roxb.)DC, Method High-performance liquid chromatography (HPLC) was utilized for this purpose with the column of HYPERSIL BDS C18 and the mobile phase containing acetonitrile and water (50:50), the detection wavelength being 287 nm. Results The average recovery rates for L-sesamin and L-asarinin were 100.13% and 1.61% and their relative standard deviation (RSD) were 101.24% and 1.46% respectively. Conclution The method can be used for the quality control of Zanthoxylum(Roxb.)DC, and the roots of this herb contains more L-sesamin and L-asarinin than its other parts do.