姜黄素对人类Burkitt淋巴瘤抗癌作用的研究

Anticancer activities of curcumin on human Burkitt′s lymphoma

目的 研究姜黄素对人类Burkitt淋巴瘤体外抗癌作用 ,探讨其抗癌作用的分子机制。方法 应用台盼蓝拒染法、MTT法、细胞周期分析法、TUNEL法及DNA片段化分析等方法 ,检测姜黄素对CA4 6细胞生长及凋亡的影响 ,并通过流式细胞术、RT PCR检测姜黄素对CA4 6细胞c myc、bcl 2、突变型p5 3、Fas蛋白和mRNA表达的影响。结果 (1)姜黄素抑制CA4 6细胞增殖呈时效及量效关系。(2 )姜黄素处理组CA4 6细胞周期发生变化 ,G0 /G1或G2 /M期细胞比例增多 ,S期细胞比例减少。 (3)姜黄素处理组CA4 6细胞发生凋亡 ,表现为TUNEL法可检测到细胞内呈棕色颗粒的凋亡细胞 ,DNA片段化分析可出现典型的梯状DNA条带。 (4)姜黄素处理组CA4 6细胞c myc、bcl 2、突变型p5 3蛋白和mRNA表达水平显著下降 ,而Fas蛋白和mRNA表达水平则上升。结论 姜黄素能够抑制CA4 6细胞增殖 ,并诱导其凋亡 ,推测可能是通过下调c myc、bcl 2、突变型p5 3,上调FasmRNA及蛋白的表达而起作用。

Objective To study the anticancer activities of curcumin on human Burkitt′s lymphoma and their molecular mechanism. Methods The effect of curcumin on the growth of CA46 cells and apoptosis were studied th rough Trypan blue exclusion, MTT assay, cell cycle, DNA fragmentation analysis a nd detection of TdT mediated dUTP nick end labeling(TUNEL). The effect of curcu min on the expression of c myc, bcl 2, mutant type p53 and Fas protein and mR NA was st udied by flow cytometry(FCM) and reverse transcription polymerase chain reactio n (RT PCR). Results 1. Curcumin inhibited proliferation of CA46 cells in a time and dose dependen t manner, 2. CA46 cells treated with curcumin showed G 0/G 1 or G 2/M phase increase and S phase decrease, 3. CA46 cells apoptosis induced by curcumin was c onfirmed by DNA fragmentation and TUNEL and 4. The expression of c myc, bcl 2 , mutant type p53 protein and mRNA was decreased sharply in CA46 cells treated w ith curcumin, while Fas protein and mRNA was increased. Conclusion Curcumin is able to inhibit the proliferation of CA46 cells and induce the cell apoptosis by down regulating the expression of c myc, bcl 2, mutant type p53 and up regulating the expression of Fas.