植物药材总DNA提取

Isolation of total DNA from Plant Chinese medicinal materials

目的 为获得高质量的 DNA,研究木质部较发达的根、根茎类以及茎木类药材的 DNA提取方法。方法 总 DNA的提取过程包括用 Tris缓冲液洗净、CTAB抽提和纯化。结果 对升麻、木、木香、乌药、芍药和木通等进行了 DNA提取 ,能较好的去除色素、多糖等干扰 PCR物质。结论 本法使用常用试剂 ,成本低 ,易于推广 ,为药材

Object To explore the method for high quality DNA extract from plant Chinese medicinal materials containing developed xylem Methods The process of DNA includes wash with Tris buffer, extract with CYAB buffer and purification mainly Results Using this method, the total DNA was isolated from Cimicifugae foelida L., Arlia chinensis L., Paeoniae lactiflora Pall.,Aucklandia lappa Decne.,Lindera aggregata (Slms) Kostern., and Akebia quinata Decne. etc This method is preferable in removing pigments, polysaccharides, substance disturbing PCR Conclusion In this process, common reagents are used, lower cost is of advantage to popularize This study provids the applying foundations for the DNA identification of plant Chinese medicinal materials