薄层色谱法分离测定青霉素V亚砜酸

Separation and determination of penicillin V sulfoxide with thin-layer chromatography

建立了测定青霉素 V亚砜酸含量的薄层色谱扫描方法 ,以 V(醋酸丁酯 ) :V(冰醋酸 ) :V(5磷酸氢二钠 ) :V(正丁醇 ) =1 2 :4:2 :1为展开剂 ,检测波长 2 85nm,线性范围 5～ 6 0 mg,最低检测限 0 .5mg.方法简便、快速、灵敏、重现性好 ,可用于青霉素

A method for separation and determination of penicillin V sulfoxide by thin layer chromatography has been established. The developer used was V(n butyl acetate):V(acetic acid):V(5% sodium phosphate):V(n butanol)=12:4:2:1. The detection wavelength was 285 nm, beam size 1.2 mm×1.2 mm, SX=5. The calibration curve was linear in the range of 5~60 mg (r=0.995, n=7) and the detectable limit was 0.5 μg. The method has been to be simple, effective, quick, sensitive and reproducible. The satisfactory results show that this method has certain practical values as an approach of quality control.