^(13)C-尿素呼气试验在蒙古沙鼠幽门螺杆菌感染检测中的应用

^(13) Curea breath test for the detection of Helicobacter pylori infection in Mongolian gerbils

目的 应用13 C -尿素呼气试验检测蒙古沙鼠幽门螺杆菌感染 ,从而建立一长期监控小型试验动物幽门螺杆菌感染无创检测技术。方法 分别在蒙古沙鼠感染幽门螺杆菌后第 2 0 ,5 0 ,10 0及 2 0 0d用13 C -尿素呼气试验进行检测 ,并对检测后沙鼠以细菌分离培养、ELISA、PCR、快速尿素酶试验、病理切片等五种常规方法检测幽门螺杆菌。结果 在上述不同检测时期 ,用13 C -尿素呼气试验所得蒙古沙鼠幽门螺杆菌感染阳性率分别为 77 78% ,83 33% ,84 2 1%和 80 0 0 % ,而应用常规方法检测结果阳性率为 83 33% ,88 88% ,94 2 1%和 85 0 0 %。比较两组试验结果发现 ,13 C -尿素呼气试验比常规检测方法检出阳性率相对偏低 ,但统计学分析发现其具有一致性。结论 13 C -尿素呼气试验用于幽门螺杆菌感染蒙古沙鼠模型检测是一种可行的无创检测方法 。

Aim To detect H pylori infection in Mongolian gerbils using 13 C urea breath test( 13 C-UBT),in order to establish an noninvasive methods to detect H pylorii infection in animal models Methods The H pylori were detected with 13 C-UBT,histology,rapid urea test,ELISA,PCR and culture on the days of 20,50,100 and 200 after the infection Results The invasive methods demonstrated sensitivity of 83 33%,88 88%,94 21% and 85 00%,but the 13 C UBT had lower sensitivity(77 78%,83 33%,84 21% and 80 00%) These data are in agreement with the results of prevalence of H pylori infection when detected by invasive methods or the 13C UBT Conclusion This method may be considered an excellent useful noninvasive for Hp infection in H pylori and it can be considered one of the important actor for H pylori infection