致人腹泻产气荚膜梭菌的分离鉴定与基因分型

Isolation,identification and genotyping of Clostridium perfringens strains causing human diarrhea

目的 研究产气荚膜梭菌与我国人群中引起食物中毒的关系。方法 从江苏扬州三家医院采集腹泻病人临床粪便样品进行细菌分离培养与生化鉴定 ,然后根据ＧｅｎｅＢａｎｋ中已发表的产气荚膜梭菌毒素基因序列 ,设计出针对ｃｐａ、ｃｐｂ、ｅｔｘ、ｉＡ、ｃｐｅ和ｃｐｂ2的 6对引物 ,应用多重ＰＣＲ方法对鉴定出的产气荚膜梭菌进行基因分型。 结果 从 40份样品中分离到 8株 (占 2 0 %)产气荚膜梭菌 ,分型结果均为Ａ型 ,其中 4株还扩增出ｃｐｂ2基因。 结论 产气荚膜梭菌是致人类腹泻的重要病原菌 ,

Aim To research the importance of Clostridium perfringens in inducing human food poisoning and diarrhea in China Method Collecting clinical stool samples from three hospitals of Yangzhou,Jiangsu Then Clostridium perfringens were isolated with culture method and identified with biochemical test Finally,the mutiple PCR was developed with six sets of primers designed based on the sequences of six Clostridium Perfringens toxin genes(cpa、cpb、etx、iA、cpe and cpb2) published in GeneBank to genotype all identified isolates Results Eight isolates were obtained and then genotyped as to be type A,and three were proved to contain cpb2 gene Conclusion Human diarrhea is significantly associated with Clostridium Perfringens type A and β2 toxin play an important role in its pathogenisis