原位RT-PCR检测革螨体内HFRSV的实验研究

Experimental study of detection of HFRSV in gamasid mites by in situ RT-PCR

目的 确定肾综合征出血热病毒 (HFRSV)在革螨体内分布和定位 ,进一步证明革螨作为HFRS传播媒介的意义。方法 采用原位RT -PCR分子杂交技术检测鼠肺组织细胞内和革螨体内的HFRSV -RNA。结果 阳性信号呈弥散分布 ,多见于鼠肺吞噬细胞、血管内皮细胞等组织细胞内 ;革螨体内的阳性信号主要分布于卵巢细胞、中肠及支囊上皮细胞中 ,前体组织细胞较少。结论 地高辛素标记的核酸探针原位RT

To study the distribution and position of virus of hemorrhagic fever with renal syndrome in gamasid mites,and ascertain gamasid mite as a transmit vector of HFRSV,in situ reverse transcription polymerase chain reaction(ISRT PCR)was used for detection of HFRSV RNA in gamasid mite The results showed that the positive signals were dispersed mostly in macrophagocyte,vascular endothelial cells of mouse lung,and in ovary cells,midgut cells of gamasid mites Specific test of dig labeled probes illuminated that the probes were specific to HFRSV and RNA