登革热抗体三种检测方法的比较

Comparison of three methods for detecting DF antibodies

目的 比较DF -IgG斑点法、胶体金免疫色谱测试法和DF -IgM斑点法检测登革热抗体的敏感性、特异性及其它优缺点。方法 用DF -IgG斑点法、胶体金免疫色谱测试法和DF -IgM斑点法对医院送检的疑似登革热病例标本进行测试 ,比较测试结果。结果 2 0 0 0年 32 2份送检标本中 ,有 4例确诊为登革热病人 ,以DF -IgG斑点法测试阳性 ,胶体金免疫色谱测试法和DF -IgM斑点法测试阳性 3例 ;在 1999年的 311份标本中 ,有 2例确诊为登革热病人 ,DF -IgG斑点法和DF -IgM斑点法测试均阳性。而用胶体金免疫色谱测试法检测 311份标本 ,阳性 18例 (包括 2确诊病例 )。结论 通过比较显示 ,DF -IgG斑点法敏感性较强 ,并具有可排除干扰因素影响的优点 ,适于在登革热散发期间使用 ;胶体金免疫色谱测试法具有快速敏感的优点 ,适于在登革热暴发流行期间作初筛试验 ;DF -IgM斑点法特异性较强 。

Aim Comparing the sensitivity,the specificity,and the valuation of three methods of detecting Dengue Fever antibody,Dengue Blot(for IgG),Dengue Fever IgM and IgG rapid immunochromatographic test,Dengue IgM Blot Method To detect IgM and IgG of sera with Dengue Blot(for IgG),Dengue Fever IgM and IgG rapid immunochromatographic test,the comparations of the results Results By Dengue Blot(for IgG)test,there are 4 positive ones in 322 samples in 2000,3 of them are both positive to Dengue Fever IgM and IgG by rapid immunochromatographic test and Dengue IgM Blot test,1of them is negtive by both test By Dengue Fever IgM and IgG rapid immunochromatographic test,there are 18 positive ones in 311 samples in 1999,while only 2 of them are positive by Dengue Blot(for IgG)test and Dengue IgM Blot Conclusions By comparing the three methods,it is rational using Dengue Blot(for IgG)as primary method,using the other two as the secondary methods when detecting DF antibodis