产超广谱β内酰胺酶肺炎克雷伯菌的检测及基因分型研究

The study of resistance and genotyping of ESBL producing Klebsiella pneumoniae

目的 对产ESBL肺炎克雷伯菌的耐药现状进行分析研究 ,对耐药株进行基因分型 ,监测耐药株的流行状况。方法 对临床分离的 195株肺炎克雷伯菌进行药敏试验和ESBL检测 ,对产ESBL菌株以随机扩增多态性DNA分型法 (RAPD)进行基因分型。结果 产ESBL肺炎克雷伯菌占 2 3% ,其中 5 1%来源于重症监护病房 (ICU)。ESBL阳性细菌对 12种抗生素的耐药性远远高于ESBL阴性菌。 2 0株产ESBL肺炎克雷伯菌分为 11型。结论 本院脑外SICU、呼吸科RICU存在产ES BL肺炎克雷伯菌的流行 ,合理使用抗生素 ,加强重点病区的消毒隔离措施 ,是控制医院感染流行的重要措施。RAPD是从分子水平对耐药细菌进行流行病学研究的一种经济、快速。

Aim To understand the current resistance and prognosticate the prevalence trend of ESBL producing K pneumoniae Methods To detect the resistance of 195 strains ESBL producing K pneumoniae and ESBL producing strains were genotyped by RAPD Result The ratio of ESBL producing strains was 23% 51% of these were originated from ICU 20 strains ESBL producing K pneumoniae were divided into 11 types Conclusion Chromosomal DNA analysis proves that some types ESBL producing K pneumoniae are epidemic in SICU,RICU The use of antibiotics reasonablly are important infection control measures RAPD is an economic,quick and credible method in epidemic analyzing