嗜热毛壳菌多糖单加氧酶的性质及协同作用研究

Study on Properties and Synergistic Effects of Polysaccharide Monopolygenase from Chaetomium thermophilum

多糖单加氧酶(polysaccharide monooxygenases, PMOs)可以氧化裂解纤维素,为纤维素酶提供更多的结合位点,对提高纤维素酶的活性具有重要意义。本研究旨在探索嗜热毛壳菌(Chaetomium thermophilum)AA9家族PMO5456的氧化方式,以及与不同类型纤维素酶的协同作用。通过对PMO反应产物进行鉴定,确定PMO5456具有C1、C4、C6位氧化。设置时间梯度,以PMO5456预处理的磷酸膨胀纤维素(PASC)为底物,测定PMO5456与嗜热毛壳菌三种纤维素酶EG1、CT2、CBH1的协同作用,结果显示随着PMO5456预处理纤维素时间的增加,纤维素酶的活性随之增加。PMO5456对三种纤维素酶EG1、CBH1、CT2的降解效率分别提高了3.6、2.2倍和60%。

Polysaccharide monooxygenases(PMOs) can oxidize cellulose to provide more binding sites for cellulase, which is important for improving cellulase activity. This study aimed to explore the oxidative mode of PMO5456 of the AA9 family from Chaetomium thermophilum and its synergistic effect with different types of cellulases. By identifying the PMO reaction products, it was determined that PMO5456 had oxidation at the C1, C4 and C6 positions. The time gradient was set, and the synergistic effects of PMO5456 and three cellulases, EG1, CT2 and CBH1, from Chaetomium thermophilum were determined by using PMO5456 pretreated with phosphoric acid-swollen cellulose(PASC) as the substrate. The results showed that with the increase of pretreatment time of PMO5456, the cellulase activity increased. The degradation efficiencies of PMO5456 for the three cellulases, EG1, CBH1, and CT2, increased by 3.6, 2.2 times and 60%, respectively.