猪瘟病毒石门株不同代次E2基因主要抗原编码区序列差异分析

Sequence analysis of the major coding sequence of E2 of different batches of hog cholera virus Shimen strains

利用 RT- PCR及序列测定对猪瘟病毒石门株不同代次毒株 E2基因主要抗原编码区及同一代次不同年代主要抗原编码区序列进行了分析 ,结果所有毒株 E2基因主要抗原编码区序列呈现较高的同源性 ,石门株不同代次毒株 E2基因主要抗原编码区核苷酸及氨基酸同源性分别为 97.7%～ 10 0 %、97.3%～ 10 0 % ;同一代次不同年代主要抗原编码区序列核苷酸及氨基酸同源性均为 98.6 %～ 10 0 %。只有 3个代次毒株发生较小的变异 ,核苷酸及氨基酸呈现 1～ 3个碱基或氨基酸的变异 ,其他代次的毒株序列完全一致。初步证实了猪瘟病毒分子结构的遗传稳定性 。

s:The major coding sequence of E2 of different batches of HCV Shimen strains and the major antigen coding region of E2 of same batches dry-frozen in different time of HCV Shimen strains were analysis by reverse transcriptase-polymerase chain reaction(RT-PCR)and sequenced.The results showed that the identity of the major coding sequence of E2 gene of all strains were very high.The identity of nucleotide and amino acid sequence of different batches of HCV Shimen trains were 97.7%～100% and 97.3%～100%.The identity of nucleotide sequence and amino acid sequence of same batches dry-frozen in different time of HCV Shimen strains were 98.6%～100%.Only 3 batches strains present low variation,1～2 nucleotide and amino acid were different.The other batches strains were identical,the identity of nucleotide sequence and amino acid sequence were 100%.It confirmed initially that molecular structure of hog cholera virus were genetically stable.It showed that the variation of hog cholera virus may be related to the selective advantage during virus transimission.