CD64及C-反应蛋白在儿童感染性腹泻中的临床意义

The clinical value of neutrophil CD64 and CRP in diagnosis of infectious diarrhea in children

目的探讨CD64及CRP在儿童感染性腹泻中的意义。方法收集96例腹泻患儿的临床资料,分为细菌性腹泻组(40例),病毒性腹泻组(36例)及非感染性腹泻组(20例);细菌性腹泻组分为轻度腹泻组(27例)和重度腹泻组(13例)。采用流式细胞术检测外周血中性粒细胞表面CD64的表达,免疫比浊法检测外周血中C反应蛋白(CRP)的水平。结果治疗前细菌性腹泻组CD64和CRP水平显著高于病毒性腹泻组及非感染性腹泻组(P<0.01);重度细菌性腹泻组CD64较轻度组高(P<0.05);细菌性腹泻患儿治疗后,CD64及CRP表达水平下降(P<0.01);治疗前CD64与CRP水平呈正相关(r=0.784,P<0.01)。结论联合检测CD64和CRP水平对儿童细菌性腹泻的早期诊断、判断病情及观察疗效具有一定的指导意义。

Objective To explore the clinical value of peripheral blood neutrophil CD64 and CRP in the diagnosis of infectious diar-rhea in children.Methods We collected the clinical data of 96 patients,who were classified into three groups:bacterial diarrhea group (n=40),viral diarrhea group (n=36)and non infectious diarrhea group (n=20);bacterial diarrhea group are subdivided into mild diarrhea group (n=27)and severe diarrhea group (n=13).The levels of peripheral blood neutrophil CD64 were measured using flow cytometry and dynamic changes of C-reactive protein were detected with immunonephelometry.Results The CD64 index and CRP levels of pre-treatment bacterial diarrhea group were significantly higher than those of viral diarrhea group and non-infectious diarrhea group (P<0.01).The CD64 index of severe bacterial diarrhea group were significantly higher than those of mild group (P<0.05).After standard treatment,the CD64 in-dex and CRP levels of severe diarrhea group decreased significantly (P<0.01).Correlation analysis showed that before treatment the CD64 index was positively correlated with CRP (r=0.784,P<0.01).Conclusion Combined detection of neutrophils CD64 and CRP contributes to the early diagnosis of infectious diarrhea,determination of the severity and evaluation of anti-infection effect.