依达拉奉联合高压氧对急性CO中毒迟发性脑病小鼠学习记忆能力的影响

Effect of edaravone plus hyperbaric oxygen on learning and memory ability of mice with delayed encephalopathy after acute CO poisoning

目的探讨依达拉奉联合高压氧对急性CO中毒迟发性脑病小鼠学习记忆能力的影响。方法通过腹腔注射CO建立急性CO中毒迟发性脑病小鼠模型。小鼠随机分成6组,从染毒后第3周开始各组使用不同方法连续治疗14 d,治疗结束后使用Morris水迷宫检测学习记忆能力,以平均潜伏期及空间探索次数来比较各组学习记忆的差异。结果 DEACMP组小鼠平均潜伏期高于对照组(P<0.05),空间探索成绩低于对照组(P<0.05);染毒后小鼠除生理盐水组其余各组平均潜伏期均低于DEACMP组(P<0.05),空间探索成绩均高于DEACMP组(P<0.05);联合治疗组平均潜伏期低于高压氧组及依达拉奉组(P<0.05),空间探索成绩高于高压氧组及依达拉奉组(P<0.05)。结论依达拉奉联合高压氧可以有效改善急性CO中毒迟发性脑病小鼠空间学习记忆能力,效果优于单纯高压氧治疗。

Objective To investigate the effect of edaravone plus hyperbaric oxygen( HBO) on learning and memory ability in mice with delayed encephalopathy after acute CO poisoning( DEACMP). Methods The animal model of DEACMP was established by injection of high-dose CO intraperitoneally in mice. Then the mice were randomly divided into six groups. From the third week after exposure,every groups were continuously treated using different methods for 14 days. After treatment,the Morris water maze equipment was used to make a behavior test of spatial memory in each group. By comparing the average incubation period and the number of space exploration,differences in learning and memory of each group were revealed. Results The average latency in DEACMP group was higher and space exploration in DEACMP group was lower than those in the control group( P < 0. 05). Apart from the saline group,the average latency in other treatment groups was lower and space exploration in other treatment groups was higher than in DEACMP group( P < 0. 05). The average latency in EPH group was lower and space exploration in EPH group was higher than in HBO group and edaravone group( P < 0. 05). Conclusion Edaravone plus HBO can improve spatial learning and memory ability in mice with DEACMP. The effect of edaravone plus HBO is better than that of HBO treatment and edaravone group.