一种酵母菌菊粉酶性质及其基因测序

Characteristics of inulinase and sequence analysis of inluinase gene from a kind of yeast strain

报道了来源于毕赤酵母 (Pichia)YI0 9菊粉酶酶学性质和该菊粉酶基因的序列分析。YI0 9菊粉酶最适反应pH值 4.5 ,最适反应温度 5 5℃ ,为外切型菊粉酶。PCR扩增YI0 9菊粉酶基因并进行核苷酸序列分析 ,该菊粉酶的基因全长 1665bp,没有内含子 ,存在 4个潜在的N

The characteristics of inluinase and sequence analysis of inluinase gene are reported in this paper. The inulinase from the yeast strain YI09 is an exoinulinase. The optimal pH and temperature are pH4.5 and 55℃, respectively. An exoinulinase gene from the yeast strain YI09 is amplified by PCR and sequenced. The result shows that the exoinulinase gene is 1?665?bp in length without any intron and contains 4 potential N glycosylation sites.