摘要
检测是认识一种病害最基本的前提,通过对三种ELISA检测GLRaV和GFLV的比较认为,双抗夹心-ELISA(DAS-ELISA)、间接-ELISA(PTA-ELISA)和A蛋白夹心-ELISA(PAS-ELISA) 都能检测GLRaV和GFLV。DAS-ELISA能缩短检测的时间,然而这项技术具有较高的株系特异性,在检测中可能产生假阴性反应,因此,必要时可用PTA-ELISA和PAS-ELISA对一些关键样品进行复检。
Comparision of three ELISA methods for detection of GLRaV showed that DAS-ELISA, PTA-ELISA and PAS-ELISA could detect GLRaV and GFLV. DAS-ELISA could shorten the detection time. The techniques , however, have higherstrain specificity and the false negatives can be caused . Therefore, PTA-ELISA and PAS-ELISA must repeatedly dectect someimportant samples.
出处
《中外葡萄与葡萄酒》
2002年第3期14-16,19,共4页
Sino-Overseas Grapevine & Wine
关键词
ELISA
方法
检测
葡萄卷叶病毒
扇叶病毒
Grape Leafroll Virus(GLRaV)
Grape Fanleaf Virus(GFLV)
ELISA detection
