摘要
为探讨甲硫氨酸在诱导高Hcy血症过程中的抗氧化作用,将Wistar大鼠随机分为正常组和1%甲硫氨酸组,喂养6周后,采用高效液相色谱法测定血清中同型半胱氨酸(Hcy)和谷胱甘肽含量(GSH),全自动氨基酸分析仪测定蛋氨酸和牛磺酸含量,转氨酶活性采用试剂盒测定。肝组织丙二醛(MDA)含量采用硫代巴比妥酸法,黄嘌呤氧化酶法和比色法测定肝组织超氧化物歧化酶(SOD)活性、总抗氧化能力(FRAP值)和还原性谷胱甘肽含量。结果表明,1%甲硫氨酸组血清Hcy和牛磺酸含量分别为3.56±0.68μmol·L-1和568.68±57.02μmol·L-1,较正常组显著升高(p<0.05)。1%甲硫氨酸组肝组织GSH含量和SOD活性分别为132.19±25.49mg·g-1和6.86±1.46U·mg-1,较正常组103.97±16.30mg·g-1和5.01±1.24U·mg-1显著升高(p<0.05)。1%甲硫氨酸组较正常组肝组织FRAP值亦升高而MDA含量降低。结果表明,甲硫氨酸在诱导高Hcy血症过程中同时具有抗氧化作用。
To investigate the effects of methionine on antioxidation,sixteen male Wistar rats were randomly divided into normal and 1% methionine groups and fed for 6weeks.Concentrations of methionine,homocysteine,glutathion(GSH)and taurine in serum were assayed by high performance liquid chromatography and amino acid analyzer respectively.Activities of alanine transaminase and aspartate transaminase were assayed.Hepatic malondialdehyde(MDA),GSH and activity of superoxide dismutase(SOD)and ferric-reducing antioxidant power(FRAP)were also measured.The results showed that serum Hcy(3.56±0.68μmol·L-1)was increased significantly after 1%Met treatment(p<0.05).Meanwhile,serum taurine(568.68±57.02μmol·L-1)and liver GSH(132.19±25.49mg·g-1)and SOD(6.86±1.46U·mg-1)were increased significantly(p<0.05).The liver FRAP value was increased and MDA was decreased after 1% methionine treatment.The results insugeted that methionine also had the antioxidation effect.
出处
《氨基酸和生物资源》
CAS
2015年第1期13-16,共4页
Amino Acids & Biotic Resources
基金
武警后勤学院院级课题(WHB201410)
