摘要
目的探讨中医平肝熄风 ,凉血泻火治法的抗神经损伤机理。方法建立谷氨酸致体外培养的大鼠海马神经元兴奋毒性损伤模型 ,并用脑溢安含药血清、细胞外调节蛋白激酶 (ERK)阻滞剂PD 980 59进行干预 ,分别检测各组神经元活化的ERK、c jun氨基末端激酶 (JNK)水平及上清液LDH活性。结果脑溢安含药血清能上调谷氨酸损伤后的大鼠海马神经元ERK水平 ,下调JNK水平 ,PD980 59能阻断脑溢安对受损经元的保护作用。结论以平肝熄风、凉血泻火为主要治法的脑溢安含药血清对谷氨酸致培养大鼠海马神经元损伤后的保护作用与MAPK信号转导途径有关 ,脑溢安上调ERK表达 ,下调JNK表达 。
ObjectiveTo explore the effects of the serum of traditional Chinese medicine Nao Yi An on glutamate induced cell death in cultured hippocampal neurons of rat and the underlying mechanisms. MethodsHippocampal neurons were cultured. The excitatory amino acid induced toxicity on cultured neurons was investigated. The viability of injured neurons was determined with the measurement of Lactate dehydrogenase (LDH) activity. Mitogen activated protein kinase (MAPK) were determined by immunoprecipitation /kinase assays /western blot detection.ResultsThe serum of Nao Yi An raised cell viability. The serum of Nao Yi An upregulated the expression of extracellular regulated protein kinases(ERK) and downregulated the expression of c Jun N terminal kinase/stress activited protein kinase(JNK) in cultured neurons. The serum of Nao Yi An induced upregulation of ERK and its anti death action were prevented with the specific ERKs inhibitor PD98059. Conclusions Activation of ERK signaling together with inhibition of JNK signaling by Chinese medicine Nao Yi An appears to be an important mechanism for its survival effects on cultured hippocampal neurons.
出处
《中国康复理论与实践》
CSCD
2002年第7期421-422,431,共3页
Chinese Journal of Rehabilitation Theory and Practice
基金
科技部 2 0 0 0度国家新药研究基金资助 (No .96- 90 1-0 5- 2 2 5)
关键词
脑溢安
谷氨酸
神经元损伤
保护作用
兴奋性氨基酸
乳酸脱氢酶
脑缺血
中药
Nao Yi An
serum pharmacology
excitatory amino acid
LDH
MAPK
cultured neurons
therapy of Traditional Chinese Medicine