口腔颌面部鳞状细胞癌病人外周血中细胞因子的检测及临床意义

The Measurement and Clinical Evaluation of Cytokines in Peripheral Blood of Patients with Oral Squamous Cell Carcinoma.

目的 :有研究证实很多细胞因子与机体抗炎、抗肿瘤功能相关 ,与一些免疫细胞的分化和生长有关。本研究通过对口腔颌面部鳞状细胞癌瘤患者外周血中IL - 2、IL - 6及TNF -α的检测 ,了解患者机体的抗肿瘤免疫状态 ,探讨这些细胞因子在肿瘤发展与转归中的临床意义。方法 :实验组选择本院口腔颌面外科住院的鳞状细胞癌患者 13人 ,平均年龄 44 .9岁 ,全部病例均有病理组织学证实。采血前 1月内未作过化疗或放疗。对照组 8人均来自健康体检自愿者。抽取受检者外周静脉血 5ml,取血清 ,用酶联免疫法 (法国CoulterCo.ImmunotechELISA标准试剂盒 )检测其中IL - 2、IL - 6和TNF -α浓度。结果采用SPSS8.0软件进行统计学分析。结果 :①实验组和对照组IL- 2分别为 (15 .38± 2 4.34 )pg/ml和 (16 .6 7± 12 .99)pg/ml,P >0 .0 5 ;IL - 6分别为 (6 .32± 5 .90 )pg/ml和 (2 .41± 3.79)pg/ml,P <0 .0 5 ;TNF -α分别为 (6 0 .71± 47.43)pg/ml和 (32 .5 0± 11.88)pg/ml,P >0 .0 5。②将鳞癌患者分为Ⅰ、Ⅱ期和Ⅲ、Ⅳ期两组 ,统计分析显示 :IL - 6在Ⅰ、Ⅱ期组与Ⅲ、Ⅳ期组 ,Ⅲ、Ⅳ期组与正常对照组间有显著性差异 ,TNF-α在Ⅲ、Ⅳ期组与正常对照组间有显著性差异。IL - 2在组间均无统计学差别。结论 :IL - 6作为分化?

Objective:To study the immune reaction by examining the immune reaction mediators-cytokines-IL-2, IL-6 and TNF -αin peripheral blood serum and explore the role of these agents in the progression and clinical manifestations of oral squamous cell carcinoma.Methods:Experiment group:13 patients with oral squamous carcinoma aged from 27 to 63 years old.Control group: 8 healthy volunteers with similar age and gender to the experiment group.The peripheral blood serum from every subject was collected and the cytokines were measured by ELISA.Results:①In cancer group and control group, IL-2 was (15.38±24.34)pg/ml and(16.67±12.99)pg/ml,P>0.05;IL-6 was(6.32±5.90)pg/ml and (2.41±3.79)pg/ml,P<0.05;TNF-αwas (60.71±47.43)pg/ml and(32.50±11.88)pg/ml,P>0.05, separately. ②IL-6 of T3,4 tumor patients was significantly higher than that of T1,2 tumor patients and control; TNF-αin T3,4 patients was statistically higher than in T1,2 patients.Conclusions:Expression of these cytokines highly correlated with the clinical status of carcinoma suggesting roles of these agents in growth regulation of the carcinoma and in the anticancer reaction.Higher IL-6 and TNF-αserum levels in patients with oral squamous carcinoma are found to be prognostic predictors of tumor development.