枯草杆菌重组修复试验和Ames试验的比较研究

Comparision between Rec assay and Ames test.

本文就枯草杆菌重组修复试验和Ames试验做了一些比较研究。结果显示两种方法对典型诱变剂的阳性检出率相当,对金属类诱变剂则以前法敏感;重组修复试验中又以芽胞法较标准划线法敏感。这种敏感性差异可能与实验原理有关。

Comparative studies of Rec assay and Ames test were carried out. The results showed that the positive rates of both methods for typical mutagens were rather the same, but for metal compounds Rec assay seemed to be more sensitive. As for the Rec assay itself, the spore method appeared to be more sensi. tive than the vegatative cell method. The likely cause of the sensitivity difference might be related to their experimental principles.