摘要
The effects of kafirins on protein and amino acid ileal digestibility have not been evaluated in vivo in pigs.The aim of this study was to determine the effects of protein profile on apparent ileal digestibility(AID)of amino acids. We used a sorghum hybrid with low tannin content(<0.5%). The same hybrid was harvested from 2 different plots with different kafirin profile. Sorghum with greater content of total kafirins had less content of y-and al-kafirins and higher content of β-and a2-kafirins than that with lower content of total kafirins. Two sorghum-soybean meal(SBM) diets were formulated: 1) low kafirin(LK) content(32.2 g/kg) and 2) high kafirin(HK) content(48.1 g/kg). A control diet(maize-SBM) and a reference SBM-diet were also prepared. The reference diet was fed to all pigs following the experimental period and was used to estimate the AID of cereals by the difference method. 'T' cannulas were fixed in the distal ileum of 18 barrows(6 by treatment), divided into 2 groups of 9 pigs. The pigs were fed 2.5 times their maintenance requirement of digestible energy(110 kcal/kg BW00.75). The AID of dry matter,protein, amino acids, and energy of the experimental diets was measured;the AID of cereals(maize, LK sorghum and HK sorghum) was estimated by the difference method. The maize-SBM diet was more digestible than the sorghum-SBM diets, only with respect to valine(P < 0.05). The AID of valine in the maize-SBM diet was higher than that in sorghum-SBM diets. The changes in kafirin profile between the diets only affected the AID of threonine(P < 0.01), which decreased by 9.5 percentage units in LK diet compared with HK diet. Regarding the AID of cereals, maize exhibited greater AID than sorghum, with respect to valine(P < 0.01) and serine(P < 0.10). A comparison of sorghum with LK and HK content showed that the AID of threonine and serine increased by 50.5(P < 0.001) and 19.2 percentage units(P < 0.05) in the latter, respec:tively. The higher content of γ-kafirins in LK sorghum negatively affected threonine and serine digestibility, implying that the AID of amino acids is affected more by the profile than the content of kafirins.
The effects of kafirins on protein and amino acid ileal digestibility have not been evaluated in vivo in pigs.The aim of this study was to determine the effects of protein profile on apparent ileal digestibility（AID）of amino acids. We used a sorghum hybrid with low tannin content（<0.5%）. The same hybrid was harvested from 2 different plots with different kafirin profile. Sorghum with greater content of total kafirins had less content of y-and al-kafirins and higher content of β-and a2-kafirins than that with lower content of total kafirins. Two sorghum-soybean meal（SBM） diets were formulated: 1) low kafirin（LK） content（32.2 g/kg） and 2) high kafirin（HK） content（48.1 g/kg）. A control diet（maize-SBM） and a reference SBM-diet were also prepared. The reference diet was fed to all pigs following the experimental period and was used to estimate the AID of cereals by the difference method. "T" cannulas were fixed in the distal ileum of 18 barrows（6 by treatment）, divided into 2 groups of 9 pigs. The pigs were fed 2.5 times their maintenance requirement of digestible energy(110 kcal/kg BW00.75). The AID of dry matter,protein, amino acids, and energy of the experimental diets was measured; the AID of cereals（maize, LK sorghum and HK sorghum） was estimated by the difference method. The maize-SBM diet was more digestible than the sorghum-SBM diets, only with respect to valine（P < 0.05）. The AID of valine in the maize-SBM diet was higher than that in sorghum-SBM diets. The changes in kafirin profile between the diets only affected the AID of threonine（P < 0.01）, which decreased by 9.5 percentage units in LK diet compared with HK diet. Regarding the AID of cereals, maize exhibited greater AID than sorghum, with respect to valine（P < 0.01） and serine（P < 0.10）. A comparison of sorghum with LK and HK content showed that the AID of threonine and serine increased by 50.5（P < 0.001） and 19.2 percentage units（P < 0.05） in the latter, respec:tively. The higher content of γ-kafirins in LK sorghum negatively affected threonine and serine digestibility, implying that the AID of amino acids is affected more by the profile than the content of kafirins.
基金
the Instituto Nacional de Investigaciones Forestales Agricolas y Pecuarias(INIFAP-México)for the financial support to the Research Project SIGI, Number 10242419454
