棉花黄萎病菌毒素对棉花作用机制的初步探讨

PRELIMINARY STUDIES ON THE MECHANISM OF ACTION WITH VD-TOXINS FROM VERTICILLIUM DAHLIAE KLEB. IN COTTON

用棉花黄萎病菌(Verticillium dahliae Kled.)T—9、SS4、VD—8、VD—5等4个菌株的毒素(VD-toxins)处理棉苗,发现各菌株的毒素都能使棉苗体内脱落酸(ABA)的含量增加,其中以T—9菌株毒素的影响最明显。各菌株毒素均可增加棉苗叶片的气孔抗性和提高叶片细胞的离子渗透率3—4倍。毒素对棉苗叶片的游离原生质体具有30—50%的损伤率。

The seedling cuttings of cotton were treated with VD-toxins from 4 isolates of V. dahliae (T-9, SS_4, VD-8, VD-5). The results suggested that the ABA content from the seedling cuttings treated with VD-toxins was significantly greater than that from those of controls, ABA content from the seedling cuttings treated with VD-toxins was significantly greater than that from those of controls, ABA content from the seedling cuttings of T-9-toxin-treated v as remarkably increased because VD-toxin content in the culture fluids from T-9 isolate was higher than that from other isolates.Stomatal resistance from leaves with VD-toxin-treated seedling cuttings was obviously higher than that from controls. The results of the ion leakage experiments indicated that electrolyte was three to four times increased from the leaves of the seedling treated with the VD-toxin solution than from the leaves of controls.The permeability from VD-toxin treated lae(?) cells of the seeding was obviousy increased controls. The free protoplasts from VD-toxin treated leaf cells of the seedlings was significantly destroied than that from double distilled water.The breaking plasma membranes was 30 to 50% greater from the free protoplasts treated with VD-toxin than that from the free protoplasts of controls.