棉株体内几种生化物质与抗枯萎病之间关系的初步研究

ON THE BIOCHEMICAL SUBSTANCES IN COTTON PLANTS RELATED TO THE RESISTANCE TO FUSARIUM WILT

棉花抗枯萎病品种86—1、陕1155,感病品种岱15、徐州142。在棉幼苗三叶期用采自河南王屯的棉枯萎菌7号小种接种,接种后12小时,各抗病品种的葡萄糖、核糖含量显著增加,而感病品种则相反。接种后抗病品种的苯丙氨酸解氨酶(PAL)活性峰出现早于感病品种,相对酶活值也高于感病品种。接种后抗病品种蛋白质含量出现先低后高趋势,感病品种则始终保持较高水平。木质素含量的增加与棉花品种抗病性存在正相关性。伴随着PAL活性的增加,木质素含量也迅速增加。结果表明,棉花品种对枯萎病的抗性与PAL活性有密切关系,并通过代谢最终产物之一的木质素的增加得到表达。

Four cotton cultivars including two cotton Fusarium wilt resistant cultivars 86-1 and Shanxi 1155 and two susceptible cultivars Deltapine 15, Xuzhou 142 were used to study for their resistance by analysing some key biochemical substances 12 hrs after inoculating with Fusarium oxysporum f. sp. vasinfectum (FOV) at 3-leaf-stage of cotton plants. The results showed that ribose and glucose were markedly increased in two resistant cultivars, while these two sugars were decreased in susceptible cultivars. Peak of phenylalanine ammonia lyase(PAL)activity in resistant cultivars appeared earlier than they were in susceptible cultivars. The activities of PAL in resistant cultivars showed a higher level. Contents of protein in resistant cotton cultivars appeared a lower level at the begining of test, however later, it became a higher level. Protein contents in susceptible cultivars remained a higher level. Lignin contents were positively correlated to the disease resistance along with increasing of PAL activities, and the disease resistance along with increasing of PAL activities, and the amounts of lignin also rapidly accumulated. As a result, the increase of biochemical substances such as ribose, glucose,protein and lignin contents after inoculation with FOV, caused at the same time the increase of activity of PAL in resistant cultivars and the higher resistance to FOV.