期刊文献+

Anticaries and antimicrobial activities of methanolic extract from leaves of Cleistocalyx operculatus L.

Anticaries and antimicrobial activities of methanolic extract from leaves of Cleistocalyx operculatus L.
下载PDF
导出
摘要 Objective: To investigate antimicrobial activities of methanolic extract of leaves of Cleistocalyx operculatus L.(C. operculatus) grown in Vietnam.Methods: The methanolic extract of C. operculatus leaves was phytochemically screened and tested for its antimicrobial activity against six Gram-positive bacteria(three of which were antibiotic multiresistant Staphylococcus spp.), two Gram-negative bacteria,and one fungal species using an agar diffusion method. Anticaries activity was tested using p H drop and biofilm assays formed in 96-well plastic plates.Results: Phytochemical screening revealed the presence of flavonoids and terpenes, in which flavonoid content was 6.8 mg/g dry material. Antibacterial activity of the C. operculatus extract was shown only against Gram-positive bacteria Staphylococcus aureus, Bacillus subtilis and Streptococcus mutans GS-5(S. mutans), and three multiresistant bacteria being Staphylococcus epidermidis 847, Staphylococcus haemolyticus 535 and Staphylococcus aureus North German epidemic strain. Interestingly, methanolic extract of C. operculatus leaves exhibited the anticaries activity against S. mutans in terms of inhibition of acid production and biofilm formation. Activity of two key enzymes responsible for acidogenicity of S. mutans, F-ATPase and phosphotransferase system were inhibited by the extract with IC_(50) of 51.0 and 98.0 m g/m L, respectively. Cytotoxicity of the extract against keratinocytes was found only for higher concentrations [IC_(50)=(119.98 ± 4.63) m g/m L].Conclusions: The methanolic extract of C. operculatus leaves has the potential for development of antimicrobial preparations, especially anticaries products. Objective: To investigate antimicrobial activities of methanolic extract of leaves of Cleistocalyx operculatus L.(C. operculatus) grown in Vietnam.Methods: The methanolic extract of C. operculatus leaves was phytochemically screened and tested for its antimicrobial activity against six Gram-positive bacteria(three of which were antibiotic multiresistant Staphylococcus spp.), two Gram-negative bacteria,and one fungal species using an agar diffusion method. Anticaries activity was tested using p H drop and biofilm assays formed in 96-well plastic plates.Results: Phytochemical screening revealed the presence of flavonoids and terpenes, in which flavonoid content was 6.8 mg/g dry material. Antibacterial activity of the C. operculatus extract was shown only against Gram-positive bacteria Staphylococcus aureus, Bacillus subtilis and Streptococcus mutans GS-5(S. mutans), and three multiresistant bacteria being Staphylococcus epidermidis 847, Staphylococcus haemolyticus 535 and Staphylococcus aureus North German epidemic strain. Interestingly, methanolic extract of C. operculatus leaves exhibited the anticaries activity against S. mutans in terms of inhibition of acid production and biofilm formation. Activity of two key enzymes responsible for acidogenicity of S. mutans, F-ATPase and phosphotransferase system were inhibited by the extract with IC_(50) of 51.0 and 98.0 m g/m L, respectively. Cytotoxicity of the extract against keratinocytes was found only for higher concentrations [IC_(50)=(119.98 ± 4.63) m g/m L].Conclusions: The methanolic extract of C. operculatus leaves has the potential for development of antimicrobial preparations, especially anticaries products.
出处 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2017年第1期43-48,共6页 亚太热带生物医学杂志(英文版)
基金 Supported by The German Academic Exchange Service(DAAD)funding for Phuong T.M.Nguyen(Reference No.91562658)in 2015
关键词 Cleistocalyx operculatus ANTIBACTERIAL ACTIVITY ANTICARIES CYTOTOXICITY Cleistocalyx operculatus Antibacterial activity Anticaries Cytotoxicity
  • 相关文献

参考文献2

二级参考文献48

  • 1王田,邱学德,黄杰,刘乔保,肖民辉,杨晓华.茶多酚对膀胱肿瘤细胞端粒酶活性的影响[J].现代泌尿外科杂志,2005,10(6):314-316. 被引量:6
  • 2Rezaeizadeh A, Zuki ABZ, Abdollahi M. Determination of antioxidant activity in methanolic and chloroformic extracts of Momordica Charantia. Afr J Biotechnol 2011; 10 (24): 4932-4940.
  • 3Afanas'ev I. Signaling and damaging functions of free radicals in aging-free radical theory, hormesis, and TOR. Aging Dis 2010; 1(2): 75-88.
  • 4Ghimire BK, Seong ES, Kim EH, et al. Comparative evaluation of the antioxidant activity of some medicinal plants popularly used in Nepal. J Med Plant Res 2011; 5(10): 1884-1891.
  • 5Uttara B, Singh AY, Zamboni P, Mahajan RT. Oxidative stress and neurodegenerative diseases: a review of upstream and downstream antioxidant therapeutic options. Curr Neuropharmacol2009; 7(1): 65-74.
  • 6Devasagayam TP, Tilak JC, Boloor KK, Sane KS, Ghaskadbi SS, Lele RD. Free radicals and antioxidants in human health: current status and future prospects. J Assoc Physicians India 2004; 52: 794-804.
  • 7Willcox JK, Ash SL, Catignani GL. Antioxidants and pre- vention of chronic disease. Crit Rev Food Sci Nutr 2004; 44(4): 275-295.
  • 8Patil SM, Kadam VJ, Ghosh R. In vitro antioxidant activity of methanolic extract of stem bark of Gmelina arborea Roxb. (Verbenaceae), Intern J Pharm Tech Res 2009; 1(4): 1480-1494.
  • 9Rahman K. Studies on free radicals, antioxidants, and co-factors. Clin Interv Aging 2007; 2(2): 219-236.
  • 10Raffa M, Atig F, Mhalla A, Kerkeni A, Mechri A. Decreased glutathione levels and impaired antioxidant enzyme activities in drug-naive first-episode schizophrenic patients. BMC Psychiatry 2011; 11: 124.

共引文献16

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部