摘要
Objective:To investigate the hepatoprotective effect of Opuntia dillenii seed oil(ODSO)on CCl_4 provoked liver injury in rat.Methods:Animals were treated orally with ODSO at a concentration of 2 mL/kg,once daily for one week before the first intraperitoneal injection of CCl_4,and thereafter the administration of the oil was continued for 7 days until the introduction of the second injection of CCl_4.Fourteen hours after the last dose of CCl_4,rats were sacrificed,and the relative liver weight,weight gain,alkaline phosphatase,aspartate amino transferase,alanine aminotransferase,direct bilirubin,total bilirubin,triglycerides,total cholesterol,very low density lipoprotein,low density lipoprotein,high density lipoprotein,plasmatic glucose,urea,creatinine,acid uric and malondialdehyde were determined.Results:The significant increase was found in relative liver weight and plasma levels of alanine aminotransferase,aspartate amino transferase,alkaline phosphatase,total bilirubin,direct bilirubin,triglycerides,very low-density lipoprotein,urea,uric acid and malondialdehyde.Likewise,the significant decrease was indicated in the weight gain and the level of glucose plasmatic,and high-density lipoprotein levels in CCl_4 produced liver injury in rats were re-established to normal levels when treated with ODSO.While,no change was observed in the total cholesterol,low-density lipoprotein and creatinine in all animals.Conclusions:We conclude that the ODSO has a protective effect on CCl_4-mediated liver injury.Hence,we suggest its inclusion as a preventive control of liver disorders.
Objective:To investigate the hepatoprotective effect of Opuntia dillenii seed oil(ODSO)on CCl_4 provoked liver injury in rat.Methods:Animals were treated orally with ODSO at a concentration of 2 mL/kg,once daily for one week before the first intraperitoneal injection of CCl_4,and thereafter the administration of the oil was continued for 7 days until the introduction of the second injection of CCl_4.Fourteen hours after the last dose of CCl_4,rats were sacrificed,and the relative liver weight,weight gain,alkaline phosphatase,aspartate amino transferase,alanine aminotransferase,direct bilirubin,total bilirubin,triglycerides,total cholesterol,very low density lipoprotein,low density lipoprotein,high density lipoprotein,plasmatic glucose,urea,creatinine,acid uric and malondialdehyde were determined.Results:The significant increase was found in relative liver weight and plasma levels of alanine aminotransferase,aspartate amino transferase,alkaline phosphatase,total bilirubin,direct bilirubin,triglycerides,very low-density lipoprotein,urea,uric acid and malondialdehyde.Likewise,the significant decrease was indicated in the weight gain and the level of glucose plasmatic,and high-density lipoprotein levels in CCl_4 produced liver injury in rats were re-established to normal levels when treated with ODSO.While,no change was observed in the total cholesterol,low-density lipoprotein and creatinine in all animals.Conclusions:We conclude that the ODSO has a protective effect on CCl_4-mediated liver injury.Hence,we suggest its inclusion as a preventive control of liver disorders.
基金
financed by CNRST, Morocco (PPR2)
