茶多酚与茶色素对大鼠肝癌前病变组织细胞周期调节因子的影响

Effects of tea polyphenols and tea pigments on cell cycle regulators in rat liver precancerous lesions

目的 利用大鼠肝癌前病变模型探讨茶多酚和茶色素对细胞周期因子的影响。方法 采用改良的Solt Farber大鼠肝癌癌前病变模型 ,分别饮用 0 1 %或 0 1 %茶多酚 ,喂养 56d。采用Westernblot方法观察细胞周期素D1、P2 1 WAF1 /CIP1 、GADD45和PCNA蛋白的表达 ;用RT PCR在mRNA水平观察Cdk4的表达。结果 茶多酚和茶色素显著抑制细胞周期素D1、Cdk4和PCNA表达 ,诱导P2 1 WAF1 /CIP1和GADD45蛋白表达。结论 茶多酚和茶色素通过调节细胞周期调节因子而诱导细胞周期阻滞 。

Objectives This study is to investigate the effects of tea polyphenols and tea pigments on cell cycle regulators in rat liver precancerous lesions. Methods The modified Solt Farber precancerous liver rat model was used. Rats were given water, tea polypheol solution (0.1%) or tea pigment solution (0.1%) throughout the whole expreriment (56 days). Cyclin D1, P21 WAF1/CIP1 , GADD45 and PCNA protein expression were detected by Western blotting and the RT PCR method was applied to study the expression of Cdk4. Results Cyclin D1, Cdk4 and PCNA expressions were significantly inhibited, and the expression of P21 WAF1/CIP1 and GADD45 were significantly induced by tea polyphenols and tea pigments treatments. Conclusion Tea polyphenols and tea pigments induced cell cycle arrest and inhibited cell proliferation by regulating cell cycle regulators.