铝染毒对大鼠海马中五种元素含量的影响

Effects of exposure to aluminium on some metal elements contents in hippocampus of rat

目的 探讨铝染毒后对大鼠海马中的锌、铁、铜、钙元素的含量的影响。方法 按大鼠体重 8%的进食量计算 ,分别在基础饲料中添加AlCl3·6H2 O ,分别按 0 (A) ,1 1 2 (B) ,55 9(C) ,1 1 1 9(D)mgAl3+ /kgBW 4个剂量组连续喂饲SD雄性大鼠 90d。然后 ,用原子吸收分光光度法测定大鼠海马中铝、锌、铁、铜和钙的含量。结果 铝经口染毒后 ,大鼠海马中铝含量明显升高 ,且有显著的剂量效应关系。对照组与 3个铝染毒剂量组脑海马中锌、铁、钙、铜含量分别为 :A组 (1 8 2 9± 2 48,2 4 86±1 97,48 69± 2 2 0 8,4 53± 0 99) μg/g ;B组 (1 7 2 2± 2 0 6 ,2 7 54± 2 87,42 79± 1 4 42 ,4 0 6± 0 41 ) μg/g ;C组 (1 4 46± 1 90 ,2 0 1 8± 2 79,2 9 95± 7 33 ,3 98± 0 2 5) μg/g;D组 (1 5 85± 2 54 ,2 0 96± 2 83 ,36 1 4± 1 2 66 ,4 53± 0 58) μg/g。与对照组比较 ,B、C组锌、铁含量均有显著性降低 ,C组的钙有显著性降低。结论 铝经口染毒会造成铝在脑海马中蓄积 。

Objective To investigate the effects of exposure to aluminum (Al) on Zn,Fe,Cu and Ca contents in hippocampus of rats Methods AlCl 3·6H 2O was administered orally through diet(8% of body weight) to SD rats at doses of 0(A),11 2(B),55 9(C),111 9(D) mg Al 3+ /kg BW for successive 90 days Then Al,Zn,Fe,Cu and Ca contents in hippocampi of rats were determined by atomic absorption spectrophotometry Results After oral exposure to Al, the Al content in hippocampus of rat increased significantly with a remarkable dose effect relationship( r =0 731, P <0 001),The Zn,Fe,Ca and Cu contents in these four groups are as follows:GroupA(18 29±2 48,24 86±1 97,48 69±22 08,4 53±0 99)mg/g,GroupB(17 22±2 06,27 54±2 87,42 79±14 42,4 06±0 41)mg/g,GroupC(14 46±1 90,20 18±2 79,29 95±7 33,3 98±0 25)mg/g,GroupD(15 85±2 54,20 96±2 83,36 14±12 66,4 53±0 58)mg/g Compared with the control group A, the Zn and Fe contents in group B and group C decreased significantly( P <0 05),and the Ca content in group C also decreased significantly Conclusion Oral exposure to Al may result in accumulation of Al in hippocampus of brain and thus affect some essential elements (Zn, Fe, Cu and Ca) contents in the hippocampus at different degrees