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菊花矮化类病毒双向电泳检测 被引量:2

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摘要 菊花矮化类病毒(CSV),是菊花的一个重要病害,引起植株严重矮化,花序变小而降低或失去其观赏价值。在1945~1947年,菊花矮化病在美国大流行,使30%~60%植株失去商品价值。此病在美国、加拿大、英国、荷兰有分布,在我国也有报道。但它在菊花生产中的重要性,它的分布及流行情况不清楚。在1987~1989年,我们在这方面做了一些工作。
出处 《植物检疫》 北大核心 1991年第3期173-174,共2页 Plant Quarantine
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  • 1Baumstark T, Schroder A R, Riesner D. 1997. Viroid processing.. Switch from cleavage to ligation is driven by a change from a tetraloop to a loop E conformation. The EMBO Journal, 16.. 599 - 610.
  • 2Candresse T, Macquaire G, Brault V, Monsion M, Dunez J. 1990. 32P- and biotin-labelled in vitro transcribed cRNA probes for the detection of Potato spindle tuber viroidand Chrysanthemum stunt viroid. Research in Virology, 141 (1): 97 - 107.
  • 3Candresse T, Macquaire G, Monsion M, Dunez J. 1988. Detection of Chrysanthemum stunt viroid (CSVd) using nick translated probes in a dot-blot hybridization assay. Journal of Virological Methods, 20 (3): 185 - 193.
  • 4陈炜 田颖川 刘勇 彭勃 夏远南 田波.用互补DNA分子杂交和聚丙烯酰胺凝胶电泳测定我国菊花矮化类病毒[J].科学通报,1981,:886-886.
  • 5Chung B N, Pak H S. 2008. Seed transmission of Chrysanthemum stunt viroid in chrysanthemum. Plant Pathology Journal, 24:31 - 35.
  • 6Diener T O, Lawson R H. 1973. Chrysanthemum stunt: A viroid disease. Virology, 51 (1): 94 - 101.
  • 7HadidiA, Flores R, Randles J W, Semancik J S. 2003. Viroids. EnfieldN H: Science Publishers: 218 - 223.
  • 8Hooftunan R, Arts M J, Shamloul A M, van Zaayen A, Hadidi A. 1996. Detection of Chrysanthemum stunt viroid by reverse transcription-polymerase chain reaction and by tissue blot hybridization. Acta Horticulturae, 432:120 - 128.
  • 9Hosokawa M, Ueda E, Ohishi K, Otake A, Yazawa S. 2004. Chrysanthemum stunt viroid disturbs the photoperiodic response for flowering of chrysanthemum plants. Planta, 220 (1).. 64 - 70.
  • 10Li R, Mock R, Huang Q, Abad J, Harttmg J, Kinard G. 2008. A reliable and inexpensive method of nucleic acid extraction for the PCR-based detection of diverse plant pathogens. Journal of Virological Methods, 154:48 - 55.

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