盐适应和非盐适应烟草愈伤组织蛋白质组分变化

Changes in Protein Patterns of Salt-Adapted and Salt-Unadapted Tobacco Callus

通过在培养基中逐代增加氯化钠浓度方法获得的适应0.5％到1.0％NaCl的盐适应细胞系,与非盐适应细胞系的蛋白质电泳图谱相比,其蛋白质SDS-PAGE图谱中出现了26kD蛋白质,但量不多;此外,40.3和16.5kD蛋白质量增加,其中16.5kD蛋白质增加显著;而33.8和18kD蛋白质则减少。上述16.5,26和40.3kD蛋白质的诱导产生或含量的增加和细胞在盐环境中表现出的适应性相伴随发生,表明这3种蛋白质可能与抗盐性有关。

Experiments were carried out with two lines of cultured tobacco callus. One of the lines was adapted to growing in a medium containing 0.5% to 1.0% NaCI by increasing the concentration of NaCl in the medium step by step, while the other line was salt-unadapted in which the callus was grown on the same medium but without added salt(control callus), or the control callus was directly exposed to the medium containing 0.75 % or 1.0 % NaCl. The protein patterns of salt-adapted cells were compared with those of unadapted cells on one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Two proteins (40.3 and 16.5 kD) markedly increased in the salt-adapted cells, in which 16.5 kD protein comprised of about 13.6%～18% of the total extractable proteins. New protein (26 kD) appeared and the other two proteins (33.8 and 18kD) decreased in amount. The protein patterns did not change during the growth cycle of the cells. The adaptability of cells to the salt-containing medium coincided with the occurrence of 26 kD protein and the increase of 40.3, 16.5 kD proteins. Our results indicate that there may be an involvement of the three proteins in the adaptation of cells to NaGl stress. It seems that the three proteins which occur during NaCl adaptation are salt-adapted proteins, not injured ones.