摘要
大豆受SO_2熏气过程中,在一定剂量作用下对SO_2抗性有提高的现象,与此同时,在叶子中出现了一个15kD蛋白,其蛋白量经一定时间增加后不再继续增加,熏气结束后即逐渐降低。纯化的15kD蛋白经SDS-PAGE和IEF检测,证明是均一的,它在280nm处的吸收很低,pH变化对紫外差吸收谱无影响,并且不含糖基;氨基酸组成分析显示,它所含脂肪族氨基酸比例很高,几乎不含芳香族氨基酸,没有发现甲硫氨酸。
When soybean seedlings were exposed to a certain dosage of SO_2, a 15 kD protein appeared in the leaves of seedlings. The level of 15 kD protein increased to some extent during fumigation, then ceased to increase after a certain period of fumigation, and gradually declined when fumigation was over. The appearance of 15 kD protein was accompanied by an increase of the resistance to SO_2. SDS-PAGE and IEF showed that purified I5 kD protein was homogeneous. Its absorbance at 280 nm was weak, and the changes of pH had no effect on UV-absorption spectrum. It was not a glycoprotein. Analysis of its amino acid composition showed that aliphatic amino acids were the predominant ones, almost no aromatic amino acids and no methionine were found.
基金
国家自然科学基金
关键词
大豆
二氧化硫
15KD蛋白
SO_2
soybean
15 kD protein
UVabsorption
glycosidation
amino acid composition
