摘要
目的 探讨珊瑚羟基磷灰石（CHAP）体外复合经扩增的骨髓基质细胞（BMSc）在大动物大段骨缺损中的修复能力及放射性核素骨显像技术在此过程中的应用价值。方法 中国青山羊6只分为实验组和对照组,每组3只。分别造成左侧胫骨2cm骨缺损,实验组缺损区植入CHAP和BMSc复合体,对照组不植入任何填充物。术后2、4、8周分别通过放射性核素骨显像进行监测。结果 放射性核素骨显像中感兴趣区（ROI）计数和摄取比值显示对照组在3个时间点均未见再血管化的表现及明显的成骨活动,而实验组则随着时间的延长再血管化的数量和成骨的质量呈现出上升的趋势。结论CHAP和BMSc复合体具有良好的修复大动物大段骨缺损的能力,放射性核素骨显像在修复过程中有非常准确的预测效果。
Objective We combined coralline hydroxyapatite porous (CHAP) with bone marrow stro-ma cell, then repaired the long - segment tibia defect monitored by 99mTc - MDP ECT with them. Methods 6 Chinese Qing caprines were divided into 2 groups. The left tibia of every caprine was made a defect of 2 cm. Experiment group were filled with CHAP and BMSc combination , and nothing to the controlled group. 99mTc-MDP ECT was used to monitor at 2,4,8 weeks separately. Results 99mTc-MDP uptakes and ratios of the region of interesting(ROI) indicated that there was no signal about the revascularization and bone regeneration in the controlled group, on the other hand they had a rising tendency in experiment group. Conclusion CHAP and BMSc combination has good ability to repair long-segment bone defect of large animals, 99mTc-MDP ECT is a effective method to monitor the repairing process.
