摘要
用乙醇逐步沉淀和离子交换柱层析方法对麸皮中制备的水溶戊聚糖(WSP)和水不溶戊聚糖 (AEP)进行分级纯化 ,并对各组分的组成进行了分析 .结果表明 ,用乙醇逐步沉淀时 ,随着乙醇体积分数的增加 ,所得分级组分具有较高的分支 ;用离子交换柱层析时 ,WSP可分级为 2个组分 ,AEP可分级为 3个组分 ,并且用NaCl较H2
In order to obtain homogenous fractions, water soluble pentosans(WSP) and water insoluble pentosans(AEP) prepared from wheat bran were fractionated by stepwise ethanol precipitation and DEAE cellulose column chromatograph. The composition of each pentosan was analyzed. The results showed that with the gradual increase of ethanol concentration, Ara/Xyl ratio of obtained fractions from WSP and AEP was raised,indicating a high branch degree of pantosans. WSP were purified into two fractions, and AEP were purified into three fractions upon DEAE cellulose column chromatography. The fractions eluted with NaCl had higher branch degree and higher molecular weight than the fractions eluted with water.
出处
《无锡轻工大学学报（食品与生物技术）》
CSCD
北大核心
2002年第4期332-335,共4页
Journal of Wuxi University of Light Industry
基金
国家"十五"重点科技攻关计划项目 (2 0 0 1BA50 1A0 4 )资助课题
关键词
小麦麸皮
戊聚糖
纯化
分级
组成分析
wheat bran
water soluble pentosan
water insoluble pentosan
fractionation
