高强度产甘油假丝酵母突变株的选育及其发酵性状

Screening Candida glycerolgeneses Mutants with High Productivity and the Fermentation Performance of the Mutants

以一株产甘油假丝酵母为出发菌株 ,采用化学诱变 ,通过玉米浆和外加无机磷组成的磷质量浓度为 3 40mg/L的磷源选择培养基 ,得到了一株发酵时间较原菌株缩短了 8h左右 ,产甘油能力 (甘油产量约为 1 40 g/L ,甘油转化率为 5 6% )和原菌株相似的突变株 ,并研究了突变株的发酵性状 .

Candida glycerolgenses was mutated by NTG. The phosphorus concentration of screening medium, in which phosphorus source consists of corn steep liquor and inorganic phosphorus, was 460 mg/L. Several mutants were screened. Compared with original strain, the fermentation time was shorten by about 8 hours, and the glycerol production was equal to original strain.