隐甲藻(Crypthecodium cohnii)悬浮培养生产DHA

Studies on Docosahexaenoic Acid Production by Suspension Culture of Crypthecodinium cohnii ATCC30556

研究了培养温度、初始 pH、碳源及氮源等对隐甲藻 (CrypthecodiniumcohniiATCC3 0 5 5 6)悬浮培养生产DHA影响 .通过正交试验得到的优化培养基组成为 (g/L) :葡萄糖 2 0 ,甘油 2 0 ,蛋白胨 5 ,KNO3 5 ,NaCl6,MgSO41 .6,KH2 PO41 .0 ,VH 0 .0 0 6,VB12 0 .0 0 1 ,另外添加 1mL/L橄榄油 ,6mL/L丙酮酸 .优化培养条件为 :5 %接种量 ,初始 pH 7.0 .黑暗条件培养 ,装液量为 5 0 0mL三角瓶装 5 0mL培养基 ,3 0℃培养 3 2h后转到 2 0℃培养直至收获 .在优化培养基配方和培养条件下 ,隐甲藻悬浮培养 64h后 ,生物量和DHA产量分别为 1 0 .78g/L和 1 .2 1 g/L .

The effects of the culture temperature, initial pH of medium, carbon source and nitrogen source on the production of docosahexaenoic acid by Crypthecodinium cohnii ATCC30556 were studied. Through orthogonal experiments, the optimum culuture medium was obtained(g/L): glucose, 20; glycerol, 20; peptone, 5; KNO 3,5; NaCl, 6; MgSO 4, 1.6; KH 2PO 4,1.0; vitamin H, 0.006; vitamin B 12 , 0.001 as well as 1 ml/L olive oil and 6 ml/L pyruvic acid.Optimum culture conditions were followings:5% inoculum,initial pH 7.0,culture in dark,50 mL medium in 500 mL flask,after culturing at 30 ℃ for 32 h, then shifting into 20 ℃ until harvesting. Under the optimal conditions, dry biomass and DHA yields were 10.78 g/L and 1.21 g/L respectively after 64 hours' suspension culture in flask.