发酵液中辅酶Q_(10)的分离纯化和定量分析

Studies on the Purification and Quantitative Analysis of Coenzyme Q_(10) in Culture

在辅酶Q10 发酵菌体提取方法筛选的基础上 ,用薄层色谱 (TLC)、紫外光谱 (UV)结合高效液相色谱 (HPLC)方法对提纯样品进行定性和定量分析 .试验结果表明 :采用丙酮悬液超声处理 ,有机溶剂萃取 5h,由薄层色谱结合紫外光谱法定量分析辅酶Q10 ,得到较精确的结果 ,为发酵法生产辅酶Q10

Coenzyme Q 10 was an important pharmaceutic functional material. The purity of the preparations of CoQ 10 was determined by TLC, UV and high performance liquid chromatography on the base of selected pretreatments of the fermentation culture with proper organic solvent extraction. The results showed as followings: adopting acetone solution ultrasonic treatment, extracting time 5 hours, then an accurate analysis result was obtained by using TLC separation and UV absorbance. They could be efficient methods for analysis of industrial fermentation production of CoenzymeQ 10 .