细叶黄芪叶肉原生质体植株再生

PLANT REGENERATION FROM MESOPHYLL PROTOPLASTS OF ASTRAGALUS TENUIS

从细叶黄芪(Astragalus tenuis)外植体愈伤组织分化出的再生苗叶片分离原生质体。原生质体培养在改良 K8p 培养基中形成了愈伤组织。增殖后的愈伤组织转入分化培养基中分化出苗。幼苗在生根培养基中长出不定根,再生成为完整植株。再生苗叶肉原生质体在 AY培养基中,种子无菌苗叶肉原生质体在改良 K8p 或 AY 培养基中均不能形成愈伤组织。较低的2,4-D 浓度有利于原生质体愈伤组织的形成和分化,过高的2,4-D 浓度对愈伤组织的形成和分化有不利的影响。

Protoplasts were isolated from leaves of regenerated plantlets of Astragalus tenuis.Calli were formed from protoplasts cultured in modified K8p medium.After calli were transferred to differentiated medium plantlets were regenerated.The plantlets rooted on a rooting me- dium.No callus was able to be obtained when mesophyll protoplasts from regenerated plant- lets were cultured in AY medium,nor when mesophyy protoplasts from seedlings were cultured in modified K8p or AY media.Lower concentrations of 2,4-D were favourable for callus formation and differentiation,while high concentrations of 2,4-D reduced the capacity of the callus growth and differentiation.