摘要
从水稻田细菌中分离并筛选出对普遍发生,危害严重的水稻白叶枯病菌有很强拮抗作用,分泌抗菌蛋白的芽孢杆菌(Bacillus spp.)。用异步培养法及平板扩散法对4属8种植物病原细菌进行抗菌活性测定,结果表明能强烈抑制 xanthomonads 和 pseudomonads 内4种细菌的增殖。选用金氏培养基 B,37℃下,经24小时振荡培养后,可用硫酸铵沉淀法从培养液中分离出较多抑制病原菌生长的蛋白。进一步研究这一蛋白质的特性和克隆此抗性基因,将是利用现代生物技术防治水稻白叶枯病等严重病害的关键。
A strain of Bacillus spp.has been screened from thousands of bacteria isolated from rice paddy field.The strain can secrete large amount of antibacterial proteins and has a strong in- hibiting activity against the pathogen of rice leaf blight disease.Eight species in 4 genera of plant bacterial pathogens were tested for the antibacterial spectrum with the antibacterial proteins and results indicate strong inhibiting the growth of 4 species bacteria of xanthomonads and pseudomonads.Large amount of antibacterial proteins have been extracted by(NH_4)_2SO_4 precipitation from overnight culture of king's B medium.Further experiments are underway to purify the antibacterial protein with chromatography and to clone the gene encoding the protein.
关键词
拮抗细菌
抗菌蛋白
水稻白叶枯病
Antagonitic bacterium
Antibacterial protein
Rice leaf blight disease
