摘要
本文通过对动物细胞培养过程中pH值和溶氧(DO)水平控制要求的分析,设计了用空气、氧气、氮气和二氮化碳四组气体关联控制pH值和DO水平的控制系统。该系统根据对瞬时pH值和DO值参数的检测,通过微处理机进行一系列的逻辑判断和具有PI调节规律的DDC增量词节计算,再经四气体关联互补计算和多重时序控制环节,综合调节四组气体电磁阀的相互开闭时间,实现对培养过程中pH值和DO水平的控制,满足动物细胞生长的需要。
A new design of interactive control scheme is presented for the regulation of pH and DO in the mass culture of animal cells. It is simply composed of four-gas (air, oxygen, carbon dioxide and nitrogen) coupling compensating calculation (CCC) and multiple-sequence-control (MSC). By a series of logic judgements of signals from sensors and direct digital calculations (DDC), a simple proportional plus integral (PI) controller can manipulate the operations of electrocegnetic valves to meet the requirements of pH and DO levels in the growth and metabolism of animal cells.
关键词
细胞培养
动物细胞
PH
溶氧
cell culture
control systems
compensation networks
sequential control
interactive control of pH and DO
compensating calculation
