五种大戟科植物种子的脂肪酸的结构鉴定

STRUCTURAL DETERMINATION OF FATTY ACID COMPONENTS IN THE SEED OILS OF FIVE SPECIES OF EUPHORBIACEAE

对西双版纳地区采集的五种大戟科植物——中平树(Mcaranga denticulata(Bl.)Muell-Arg.)、白楸(Mallotus paniculatus(Lam.)Muell-Arg.)、木油桐(Aleurites montana(Lour)Wis.)、叶轮木(Ostodes paniculata Bl.)及滑桃树(Trewia nudiflora L.)种子中的脂肪酸作了成分分析。长键脂肪酸样品与2-氨基-2-甲基丙醇(AMP)缩合,生成2-取代-4,4-二甲基二氢(口恶)唑(DMOX)衍生物,再进行色谱-质谱分析。根据质谱中特征峰的质量,可以明确地确定脂肪键上双键及其它取代基的位置。这一方法简便有效,重现性好,能在一次分析中同时给出各成分的定性与定量结果,适合于油脂化学中脂肪酸的鉴定。

The fatty acid components of the seed oils of five species of Euphorbiaceae collected in Xishuanbanna district,Yunnan province—Aleurites montana(Lour.)Wis.,Mallatus paniculatus (Lain.)Muell-Arg.,Ostodes paniculata Bl.,Mcaranga denticulata(Bl.)Muell-Arg.,and Tre- wia nudiflora L.-have been analyzed.Long-chain fatty acids were first converted to corres- ponding 2-substituted 4,4-dimethyloxazoline derivatives(DMOX)by condensing with 2-amino- 2-methylpropanol(AMP)and subsequently analyzed by GC-MS.The position of unsatura- tion(double and triple bonds)and substituents(e.g.,hydroxyl)in the aliphatic chain can easily be located by interpreting the mass spectra recorded in this way.The qualitative as well as quantitative results can be provided for all constituents in a single GC-MS run.This me- thod proves simple and efficient,with good reliability,and is well suitable for the structure determination without reference specimens.