5′-磷酸吡哆醛高分子衍生物的辅酶功效

Study on Coenzyme Function of Macromolecular Derivatives of 5′-pyridoxal Phosphate

5′-磷酸吡哆醛(PLP)与经β-硫酸酯乙砜基苯胺活化的聚乙二醇(PEG)或右旋糖酐(Dx)共价结合,得可溶性5′-磷酸吡哆醛衍生物。用PLP-PEG和PLP-Dx衍生物代替PLP作为谷氨酸脱羧酶的辅酶,谷氨酸脱羧酶活力回复率达18%左右;用甘氨酸封闭的PLP-PEG代替PLP作为辅酶,谷氨酸脱羧酶活力回复率达44%。

Soluble derivatives of 5′-pyridoxal phosphate are prepared by covalent combination of 5′-pyridoxal phosphate with polyethylene glycol or dextran previously activated by beta sulfate ethyl sulfony aniline. The rate of GAD activity recovery for PLPDx and PLP-PEG used as coenzyme is about 18% and that for glycylated PLP-PEG is about 44%.