摘要
本文首次报道喜温植物黄瓜(Cucumis Sativus L.)幼苗在低温锻炼后,SOD、过氧化氢酶、过氧化物酶和ATP酶活性的动态,揭示在低温锻炼后,前三种酶活性显著提高,并在逆境胁迫时保持稳定,提高了它们对氧自由基和过氧化物的清除作用。同时在低温锻炼后质膜ATP酶获得耐低温性能。这些变化的总效应是使植物细胞的膜结构和物质代谢过程免遭低温逆境的破坏,为喜温植物的抗寒锻炼机理提供了实验证据。
The changes in activities of SOD, peroxidase, catalase and ATPase in chilling sensitive cucumber (Cucumis sativus L.) seedlings using biochemical and cytochemical methods were studied. The results indicated that the activities of SOD, peroxidase and catalase enhanced dramatically in cold acclimated cucumber seedlings and the three enzymes remained stable under chilling stress. Consequently, the ability of cleaning up free radical of oxygen and peroxidates increased. The cold-tolerant character of plasmalemma ATPase activity was developed after low-temperature acclimation. All these changes provided the possibility for protecting the stability of membrane structure and metabolism from chilling injury, and for the enhancement of cold tolerance by low temperature acclimation.
关键词
黄瓜
抗寒锻炼
酶活性
Cold acclimation
SOD
Peroxidase
Catalase
ATPase
Cucumber
